To study the impact of specific DDR kinase activity on lamin levels, we selectively inhibited ATM, ATR and DNA-PK using small-molecule chemical inhibitors in two independent human cell lines—a breast cancer cell line (MDA-MB-231) and a fibrosarcoma cell line (HT1080)—and assessed lamin A/C protein levels after 48 h of treatment by Western analysis. The gene discussed is PRKDC; the disease is breast cancer.