The NUP98-KDM5A chimeric oncoprotein is exclusively found in young pediatric AML patients with high risk and poor prognosis.6 Here, we describe the generation and characterization of the H9 iNK5A cell line, a human Embryonic Stem Cell (hESC) model with doxycycline-regulated expression of NUP98-KDM5A fusion protein, allowing the temporal control of NUP98-KDM5A during hematopoietic differentiation from hESC, to recapitulate and study the leukemogenic process that occurs in these patients during embryonic development. Here, KDM5A is linked to acute myeloid leukemia.