The NUP98-KDM5A chimeric oncoprotein is exclusively found in young pediatric AML patients with high risk and poor prognosis.6 Here, we describe the generation and characterization of the H9 iNK5A cell line, a human Embryonic Stem Cell (hESC) model with doxycycline-regulated expression of NUP98-KDM5A fusion protein, allowing the temporal control of NUP98-KDM5A during hematopoietic differentiation from hESC, to recapitulate and study the leukemogenic process that occurs in these patients during embryonic development. This evidence concerns the gene NUP98 and acute myeloid leukemia.