The combined use of Huaier extract and TAM in vivo can enhance the inhibitory effects of TAM on the growth of subcutaneous tumors of MCF-7 breast cancer cells and synergistically induce autophagy and apoptosis in ER+ breast cancer cells via suppression of the AKT/mTOR pathway (Qi et al., 2016). The gene discussed is ESR1; the disease is breast carcinoma.