The relative expression levels of proinflammatory cytokines (IL-6, IL-8, IL-17A, and iNOS) in the liver and spleen were markedly increased in the challenged group (INF) compared to the other treated groups because Ajwain extract (AJE) supplementation significantly alleviated the inhibitory effect of Salmonella infection. The gene discussed is CXCL8; the disease is Salmonella Infections.