Interestingly, CD8α+ iNK T cells, an effector/inflammatory subset of iNK T cells, were significantly increased in spleen, and locally in liver in mice developing GVHD (p = 0.0952, p = 0.0952, respectively), with a reciprocal decrease in CD4+ iNK T cells, regulatory subset of iNK T cells (p = 0.0952, p = 0.0952, respectively), compared to mice with BMT only. This evidence concerns the gene CD8A and graft versus host disease.