In this study, we first found that Drosophila lncRNA-CR33942 can promote the Imd signaling pathway using RNA sequencing, and then, we quantified the expression levels of AMPs Dpt and AttA in lncRNA-CR33942-overexpressing flies, lncRNA-CR33942 knockdown flies, and lncRNA-CR33942 + lncRNA-CR33942-RNAi co-overexpressing flies after Escherichia coli infection. This evidence concerns the gene ADSL and escherichia coli infection.