IL10 and major depressive disorder: SCF, IFN-γ, IL-10, and BLyS, alongside reported type 2 symptoms chronic fatigue, depression, and sleep disturbances, probable SLE (SLE-CSQ score ≥4) based on the SLE-CSQ questionnaire, as well as ACR criteria arthritis, photosensitivity, immunologic criteria, and ANA positivity differentiated unaffected Rel vs. relatives who would transition to SLE prior to disease classification.