Since the release of the apoptosis-inducing serine proteases and the production of pro-inflammatory cytokines reflect the cytolytic function of lymphocytes, we assessed the release of GrB and IFN-γ in response to CRC cells expressing high and low levels of mHsp70 in a double-color FluoroSpot assay. This evidence concerns the gene GZMB and colorectal carcinoma.