We thus compared two strategies for targeting human colorectal adenocarcinoma LS174T and LoVo cell lines that differ in their mHsp70 expression pattern providing isogenic sublines as ‘high’ and ‘low’ mHsp70 expressors: ex vivo stimulation of human NK cells from healthy donors with the 14-mer Hsp70 peptide TKD and low-dose IL-2 and genetic engineering of T cells of the same donors with a CAR targeting mHsp70. This evidence concerns the gene IL2 and colorectal adenocarcinoma.