To investigate whether T cells can be redirected against mHsp70-positive tumor cells to utilize their demonstrated long term persistence and activity, we designed a CAR targeting mHsp70 by fusing an Hsp70-binding scFv domain derived from the cmHsp70.1 mAb (46) linked to CD28 TMD by a CD8α HR and an intracellular domain containing CD28 co-stimulatory and CD3ζ signaling moieties. The gene discussed is CD28; the disease is neoplasm.