In the present study, a series of complement components were downregulated, including C1qa, C1qb, C1qc, C1ra, C1sa, C2, C3, C4b, C4bpa, C5, C8a, C8b, C8g, C9, Cfb, Cfd, Cfh, Cfi, Cfp, Masp1 and Mbl1, and a large number of immunoglobulins were downregulated, indicating the decline of humoral immune function in thyrotoxicosis mice, such as resistance to Staphylococcus aureus infection. The gene discussed is C4B; the disease is staphylococcus aureus infection.