In the present study, the abundance of Biotinidase (Btd), Transcobalamin II (Tcn2), vitamin D binding protein (VDBP, Gc), Afamin (Afm), Vitamin K-dependent protein C (Proc), and Vitamin K-dependent protein Z (Proz) were downregulated in thyrotoxicosis mice. Here, BTD is linked to thyrotoxicosis.