NOS2 and obesity due to melanocortin 4 receptor deficiency: The macrophage infiltration (% CD11b+F4/80+ of viable cells) was similar between tumors from lean and obese mice, but obesity was associated with an increased proportion of mixed M1-M2-type (% CD206+NOS2+ of CD11b+F4/80+ cells, Supplementary Figures 4A, B).