CASP3 and neoplasm: Figure 7(c) showed the pictures of tumor after peeling. Moreover, we found that nude mouse body weight in the osthole-treated group had no significant change compared with the control group (Figure 7(d)). Further, western blotting analysis revealed that osthole increased the protein expression of cleaved caspase-3 and GSDME-N and decreased the NQO1 in protein expression (Figures 7(e) and 7(f)).