To verify the role of RAB42 in HCC in vitro, we first analyzed RAB42 mRNA expression levels in liver cancer cell lines, including SMMC7721, Huh7, MHCCLM3, MHCC97L, Hep3B, HepG2 and normal hepatic cell line, LO2 (Figure 10A). This evidence concerns the gene RAB42 and hepatocellular carcinoma.