Therefore, our analysis showed that MCM6 might repress the tricarboxylic acid cycle by inhibiting the expression of NDUFAF1, inhibit aerobic glycolysis by inhibiting the expression of LDHD, and regulate the classical complement pathway activation by regulating the expression of SERPING1. DTL, ZWINT, and MCM2 were increased with MCM6 in HCC. The gene discussed is NDUFAF1; the disease is hepatocellular carcinoma.