Cell Counting Kit-8 (CCK-8 assay) and transwell assay revealed that TMBIM6 knockdown suppressed proliferation and invasion in Huh7 and PLC cells strongly (all p < 0.05), PRDX1 knockdown showed less effect, and EEF2 knockdown could not affect HCC cell proliferation or invasion (Figures 3D–F). This evidence concerns the gene EEF2 and hepatocellular carcinoma.