Conversely, the efficiency of overexpression of FOXP4-AS1 by the pcDNA3.1-FOXP4-AS1 plasmid in three thyroid carcinoma cells was also verified by RT-qPCR (Figure 2D), and overexpression of FOXP4-AS1 remarkably inhibited growth (Figures 2E, F). Here, FOXP4 is linked to thyroid gland carcinoma.