Firstly, univariate analysis identified gender, T-stage, lymph node metastasis, nerve invasion, portal vein invasion, preoperative total bilirubin, Bismuth staging, CA19-9, NLR, LMR, CD3+ T cells, and CD8+ T cells as factors influencing the prognosis of HCCA patients after radical resection (Table 3). The gene discussed is CD8A; the disease is metastatic malignant neoplasm in the lymph nodes.