Modulation in other pathway elements (such as reduction in p-mTOR Ser 2448, Ser 2481, and Thr 2446, and total mTOR protein; increase in p-4E-BP1 Thr 37/46 and total 4E-BP1 protein levels) upon exposure to fibroblast-CM was consistent between PTEN-competent (HCT116 Parental, left panel) and PTEN-loss (HCT116 PTEN-/-, right panel) isogenic CRC cells (Supplementary Figure S5C). This evidence concerns the gene PTEN and colorectal carcinoma.