To investigate whether CRIPTO plays a functional role in regulating NSCLC stemness, we modulated CRIPTO levels in SCC1 spheroids through shRNA-mediated silencing (Figure 2A, CRIPTO KO) or through exogenous expression of human CRIPTO driven by a lentiviral construct (Figure 2A, CRIPTO over). This evidence concerns the gene CRIPTO and non-small cell lung carcinoma.