For investigating the effect of TMEM44‐AS1 on promoting 5‐FU resistance in GC through its sponging effect on miR‐2355‐5p, this study conducted rescue assays using pre‐miR‐2355‐5p and anti‐miR‐2355‐5p following si‐TMEM44‐AS1 treatment. This evidence concerns the gene TMEM44 and gastric cancer.