TGF β1and αSMA expression levels as markers for hepatic fibrosis markers were significantly upregulated (P ≤ 0.01) in rat groups given 0.5 and 1 mg AgNPs while they were non-statistically significant in rats given 0.25 mg AgNPs as compared to the control group (Fig. 6a, b). Here, ACTA1 is linked to Hepatic fibrosis.