Then, we compared whether other important CCL family members produced by early OCPs could also be impacted by CRC microenvironment, qRT-PCR analysis demonstrated that the expression of CCL7 upregulated most significantly in early OCPs after treated by MC-38 CM compared to that of CCL2 and CCL6, indicating early OCPs dominantly produced CCL7 after stimulated by MC-38 secreta (Fig. 3B). The gene discussed is CCL7; the disease is colorectal carcinoma.