IL18 and myocardial infarction: Additionally, RT-PCR results shown that the transcription level of NLRP3, N-GSDMD, ASC, and IL-1β mRNA were notably upregulated in the myocardium tissues of MI/RI mice compared with those of sham, and all doses of GEN significantly suppressed the mRNA level of NLRP3, ASC, IL-1β, and IL-18 (Fig. 3A–D).