The RSPO fusion-positive tumours were subjected to whole-exome sequencing (WES).<h4>Results</h4>Our analysis identified 29 CRCs with RSPO fusions (2.8%), consisting of five with an EIF3E-RSPO2 fusion and 24 with PTPRK-RSPO3 fusions. Here, RSPO2 is linked to neoplasm.