Furthermore, and potentially of greatest importance, TWIST1 expression levels in AOR were also positively associated with the severity of CAD as assessed by Duke CAD index (P = 0.012), and with non-significant trends also for SYNTAX score (P = 0.12) and the number of diseased coronary vessels (P = 0.065) (S3 Table). Here, TWIST1 is linked to coronary artery disorder.