Based on this, we analyzed the role of HMGB3 in tumors by bioinformatics techniques through public databases by pan-cancer analysis, as well as explored the methylation of HMGB3 in different tumors and the immune profile of HMGB3 in colorectal cancer, and verified by qRT-PCR, WB and scratch assays that HMGB3 may play a role in tumor development through the wnt/signaling pathway. Here, HMGB3 is linked to neoplasm.