The aligned methylation data of the 71 hub genes in IDD were obtained from GSE129789, and our analysis indicated significant hypermethylation of ARHGAP27, CSNK2A1, ETV6, FNBP1L, KLF4, LTF, MGAM, MYO1F, NUCB2, RNF19B, and WWP3 (Figure 8F). Here, NUCB2 is linked to intervertebral disk degenerative disorder.