The methylation analysis of the hub genes showed that 11 genes (ARHGAP27, CSNK2A1, ETV6, FNBP1L, KLF4, LTF, MGAM, MYO1F, NUCB2, RNF19B, and WWP3) were hypermethylated in IDD. This evidence concerns the gene KLF4 and intervertebral disk degenerative disorder.