In clinical samples, seven (MCM2, CDK1, MCM7, MCM5, MCM6, LIG1, and MCM3) of these eight proteins were found to be significantly overexpressed in ESCC patients, with MCM2 and CDK1 as the top-2 most upregulated proteins (Figure 4G). The gene discussed is LIG1; the disease is esophageal squamous cell carcinoma.