To further investigate the function of HK2 in human ovarian cancer cells, exogenous HK2 was stably overexpressed in OVCA433 (OVCA433-GFP and OVCA433-HK2, Figure 2A) and SKOV3 (SKOV3-GFP and SKOV3-HK2, Figure 2D) cells; conversely, endogenous expression of HK2 was knocked down by stably transfecting shRNA plasmids in A2780 (A2780-shCtr and A2780-shHK2, Figure 2G) cells. Here, HK2 is linked to ovarian cancer.