These included biomarkers which are normally associated with liver disease, such as alanine aminotransferase (ALT), alkaline phosphatase (ALP), and bilirubin, confirming the possibility of a role for the liver-brain axis in NLDs such as AD, as previously reported (Bassendine et al., 2020; Jakhmola-Mani et al., 2021). This evidence concerns the gene GPT and Alzheimer disease.