This study characterizes the molecular composition of desmosomes and extradesmosomal contacts in different epidermal layers with a focus on the pemphigus autoantigens Dsg1 and Dsg3 as well as changes observed in PV patient skin using immunostaining in combination with super resolution stimulated emission depletion (STED) microscopy. The gene discussed is DSG3; the disease is acquired polycythemia vera.