Our data suggest that ACh has distinct effects associated with fungal allergen-induced pulmonary inflammation, on the one hand, promoting ILC2 cytokine production and eosinophilia, and on the other hand, inhibiting neutrophilia via suppression of CXCL1 and CXCL2 expression. The gene discussed is CXCL2; the disease is Increased total eosinophil count.