NOS2 and cancer: There were 13 cancer-immunity cycle–associated genes with an optimal λ value, including five chemokine and chemokine receptor family members (CCL11, CCL19, CCL22, CCL28, CXCR5), three immune checkpoint genes (IDO1, LAG3, TIM4), three HSP70 family members (HSPA1A, HSPA8, HSPA9), and two cytokines (NOS2 and TGFβ1).