In this study, we found the relationship between USP7 and Keap1, and demonstrated that USP7 interacts with Keap1 to reduce abnormally elevated Keap1 protein to a stable state through the proteasome degradation pathway, which may be related to the release of Nrf2 into the nucleus to trigger antioxidant responses, thus reducing ROS production and alleviating myocardial ischemia/reperfusion injury. The gene discussed is USP7; the disease is myocardial ischemia.