In summary, we found (1) a commensal relationship between PN and MES GSCs, wherein the latter gain tumorigenic benefit from PN GSCs; (2) identified that TF and DA, as humoral factors secreted from PN GSCs, enhance iron uptake by MES GSCs; (3) demonstrated that both GBM subtypes are susceptible to ferroptosis, with DA inducing ferroptotic vulnerability in the iron-addicted MES GBM. Here, TF is linked to glioblastoma.