In addition to the anatomical expression profile, a set of paraffin-embedded GBM patient tissues were retrospectively obtained from a tissue bank and further assayed for intra-tumoral differences in the expression of genes of interest, confirming the association of PN regions with DRD5 and TF as well as TfR1 expression in MES regions (Fig. 2c, d). This evidence concerns the gene TF and glioblastoma.