HES1 and posterior cortical atrophy: Immunoblotting data demonstrated that miR-628 overexpression downregulated the expression of Notch ligand, JAG1, and also downregulated the expression of Notch1, Notch2, Notch3, and Notch downstream marker HES1 in both LNCaP and PC3 cell lines (Fig. 5E) suggesting that miR-628 targets JAG1 and inhibits Notch signaling in PCa cells.