EHMT2 and acute lymphoblastic leukemia: To provide further evidence of induction of autophagy by G9a inhibitors in T-ALL cells, we performed acidotropic staining with LysoTracker Red dye and observed a significant increase in the number of acidic vesicles, indicative of autophagosome/lysosome fusion in cell lines (Fig. 6D), a process reverted with the addition of Bafilomycin A1 a known inhibitor of autophagosomes and lysosome fusion (Supplementary Fig. 8A).