EHMT2 and acute lymphoblastic leukemia: We then analyzed acid-extracted nuclear histone fractionated proteins and whole-cell lysates derived from six T-ALL cell lines treated with increasing concentrations of G9a inhibitors, DMSO or the Notch Inhibitor Compound E as a negative control by western blot, confirming a decrement of H3K9me2 (Fig. 3D, Supplementary Fig. 3A–F).