In this research, we found that gut dysbiosis caused by antibiotic exposure resulted in enrichment of intestinal Proteobacteria and elevation of gut permeability and intratumoral LPS, promoting prostate cancer proliferation and docetaxel chemoresistance via NF-κB-IL6-STAT3 axis in C57BL/6J mice. This evidence concerns the gene IL6 and Familial prostate cancer.