According to the IHC/IF staining of the tumor samples obtained at 6 h, 24 h, 72 h and 7d after RFA, we found significant changes with time manner in different groups on the pathological findings including HSP70, CD31, SMA, Ki-67, c-Met, IL-6, VEGF, and HIF-α (Figs. 3, 4 and 5). This evidence concerns the gene SMN1 and neoplasm.