The specific immunohistochemistry (IHC) or immunofluorescence (IF) staining was used to detect the changes of the tumor microenvironment, containing the expression of the following markers, HSP70 (ab181606, Abcam, Cambridge, MA), SMA (ab32575, Abcam, Cambridge, MA), Ki-67 (12202S, Cell Signaling, Danvers, MA), VEGF (AF469, R&D Systems), CD31 (77,699 T, Cell Signaling, Danvers, MA), HIF-α (MAB1536-SP, R&D Systems), IL-6 (AF-406-SP, R&D Systems) and c-Met (AF527, R&D Systems). The gene discussed is MKI67; the disease is neoplasm.