IHC staining revealed that the expression of c-Met (38 ± 3% vs 15 ± 3%), HIF-α (38 ± 4% vs 20 ± 3%), Ki-67 (50 ± 4% vs 27 ± 4%) and CD31 (23 ± 3% vs 12 ± 3%) in the tumor were decreased at 7 days in the RFA + PHA group compared to the RFA group (P < 0.001 for all comparisons) (Fig. 6-C). This evidence concerns the gene PECAM1 and neoplasm.