BALB-HER2 transgenic model express rat HER2 and represents human HER2pos BC, which spontaneously develop pre-invasive mammary lesions and progresses to invasive tumors and metastasis.35 The TUBO cells were cloned from a BALB-HER2 transgenic mouse mammary tumors and grew progressively in the mammary gland of wild type BALB/c mice.36 These two models were used to test the antitumor efficacy of HER2-DC1 i.t. combined with anti-rat HER2 antibodies 7.16.4 and 7.9.5 that mimic trastuzumab and pertuzumab, respectively. This evidence concerns the gene ERBB2 and breast cancer.