The result of trim and filling method supplemented 9 additional studies to control publication bias, showed that the expression level of mucin family was still related to the prognosis of patients with pancreatic cancer (HR = 1.24,95%CI 1.01–1.52), which further proved that the impact of publication bias on this study was not significant, and the results of our study had high credibility. This evidence concerns the gene MUC5AC and familial pancreatic carcinoma.