Our optimized pipeline to deliver this suite of CRISPR tools was applied to modulate endogenous gene expression of Rab11 family members, demonstrating that re-activation of endogenous Rab25 expression can promote the invasive migration of cancer cells, and revealing new insight into the localization and functions of Rab11a, Rab11b, and Rab25. The gene discussed is RAB11B; the disease is cancer.