To evaluate whether the putative gatekeeper mutation blocks the effects of MRX-2843 in NSCLC cells, a derivative of the 633 NSCLC cell line stably expressing an shRNA targeting the untranslated region of MERTK (633-shMERTK) (36) was transduced with plasmids driving ectopic expression of the L593G predicted gatekeeper mutant protein, a kinase-dead K619R mutant protein (37), or GFP as a control (Figure 4B). This evidence concerns the gene MERTK and non-small cell lung carcinoma.