Although CD8+T cells can directly kill cancer cells, some other regulatory immune cells, such as MDSCs and Tregs, play important roles on regulation of antitumor activity of CD8+T cells.34, 35FACS results showed that the percentages of MDSCs in MDSC-DCs-treated-groups were only slightly lower than that of the PBS group, but there was no statistical difference (Fig. 3CandSupplementary Fig. S4C[available in the online version only]), suggesting that inhibition of MDSCs might not contribute to the enhanced antitumor activity of CD24+MDSC-DCs. This evidence concerns the gene CD8A and cancer.