Subsequently, we used western blot analysis to detect normal lung epithelial cell lines HBE and NSCLC cell lines A549, H1299, H358, SPCA1, PC9, HCC827, and H1993, among which lung adenocarcinoma cell lines are A549, H1299, SPCA1, and H1993. The gene discussed is ATP2C1; the disease is lung adenocarcinoma.