The effects of PFKM, ADGRG1, ALDOA, GAK, RDH11, TJP3, TPM3, TPT1, and VDAC1 on the prognosis of HNSCC and the immunological changes in the HNSCC microenvironment have rarely been reported and need further investigation. This evidence concerns the gene VDAC1 and head and neck squamous cell carcinoma.