In the present study, we found that the downregulation of UNC13B could in turn downregulate MAP3K7, CDK4, and PINK1 in K-562 cells; hence, UNC13B might directly or indirectly promote the biological activity of ATO-resistant CML cells by affecting the expression of the abovementioned proteins. Here, CDK4 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.