Moreover, treating the ESCC cell lines with both SP2509 and UNC0642 for 1-3 days increased both the di-methylation levels of Lys4 in histone H3 (H3K4me2) and the di-methylation levels of Lys9 in histone H3 (H3K9me2) (Figure 1(i)), suggesting that these inhibitors' effects on the viability of ESCCs are mediated predominantly by inhibiting LSD1. The gene discussed is KDM1A; the disease is esophageal squamous cell carcinoma.