In addition, by using NF-κB p65 transcription factor assay, we further found that TBrC, T (50 μg/mL) and a positive control Bay (0.62 μg/mL) also inhibited TNFα-induced nuclear transcriptional activation of NF-κB p65 in human lung cancer A549 cells (Figure 5D). The gene discussed is TNF; the disease is lung cancer.