The aims of the present study were to develop a mouse model of tEAEC infection using C57BL/6 adult mice orally inoculated with the archetype EAEC 042 strain, to characterize EAEC infection in vivo and in situ, to evaluate the expression of ZO-1 tight junctions and β-catenin adherence junction and the role of AAF/II fimbriae in β-catenin and MUC1 expression during infection. Here, TJP1 is linked to infection.