In order to address its beneficial action as an anti-inflammatory molecule in knee OA synovitis, we aimed to determine whether CLU has a protective effect against IL-1β-induced inflammation in knee OA FLSs through counteracting activation of PI3K/Akt signaling pathway and to investigate its mRNA and protein levels in the systemic and local joint environment of knee OA patients with and without synovitis. This evidence concerns the gene AKT1 and synovitis.